Table 4 PCR primers for the generation of recombinant plasmids
Name of Primers | Sequence of primer (5′ → 3′) | Product size(bp) | Target gene |
|---|---|---|---|
CSFV-W-F | GAGGTTAGTTCATTCTCGTATACACGA | 310 | 5′UTR |
CSFV-W-R | TATCAGGTCGTACTCCCATCAC | ||
CSFV-V- F | CCCTTCACAACCTTACCCGACTGATTG | 295 | NS5B |
CSFV-V- R | CAGGCCTGAACCTGAGCTGGTGAAC | ||
ASFV-F | AGTTATGGGAAACCCGACCC | 257 | p72 |
ASFV- R | CCCTGAATCGGAGCATCCT | ||
APPV-F | CGCGGATCCACAGCCTACTGATGATCAGTCGATG | 336 | 5′UTR |
APPV-R | CCGGCACTCTATCAAGCAGTAAGGTC |