Fig. 4

IFITMs overexpression inhibits the late step of PFV replication. A, B The pcPFV was co-transfected into HEK293T cells with IFITM eukaryotic expression plasmids or control vector, respectively. At 48 h post-transfection, the PFVL indicator cell lines were co-incubated with cell culture supernatants (1/2) or transfected HEK293T (1/20) cells. The level of luciferase was measured 48 h after co-incubation. Values were statistically evaluated using one-way ANOVA. Compared with the pcPFV and control vector co-transfection group: *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. Error bars represent the standard deviations. C The level of PFV protein expression in transfected HEK293T cells. D–F We performed the above experiments in HT1080 cells. Data are representative of three independent experiments