Fig. 1

IFITM3 inhibits PFV entry into HT1080 cells. HT1080 cells were infected with the PFV virus stock solution (2 MOI) 24 h after transfection of IFITM3 expression plasmid or control vector, and total DNA was extracted 30 h after infection. The semiquantitative PCR (A) and real-time PCR (B) results indicate the level of proviral DNA integration. C The levels of PFV entry were evaluated as described in the Materials and Methods. The amount of PFV Gag gene relative to gapdh indicates the level of viral mRNA. Data are representative of three independent experiments. Values were statistically evaluated using a two-way ANOVA. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. Error bars represent the standard deviations