Test kits | Nucleic acid volume (μL) | Internal standard | Number of cycles | Minimum detection limit (copies/mL) | Target genes | Test result positive | |||
---|---|---|---|---|---|---|---|---|---|
Maccura kit | 20 | Yes | 40 | 450 | ORF1ab / N / E | ORF1ab gene Sigmoidal amplification curve and Ct ≤ 38 | N gene Sigmoidal amplification curve and Ct ≤ 38 | E gene Sigmoidal amplification curve and Ct ≤ 37 |  |
 +  |  +  |  +  | positive | ||||||
 +  |  +  | − | positive | ||||||
 +  | − |  +  | positive | ||||||
Sansure kit | 20 | Yes | 45 | 200 | ORF1ab / N | ORF1ab gene Sigmoidal amplification curve and Ct ≤ 40 | N gene Sigmoidal amplification curve and Ct ≤ 40 |  |  |
 +  |  +  |  | positive | ||||||
 +  | − |  | positive | ||||||
− |  +  |  | positive |