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Table 1 General information of the two kits

From: Comparison of the performance of two real-time fluorescent quantitative PCR kits for the detection of SARS-CoV-2 nucleic acid: a study based on large real clinical samples

Test kits

Nucleic acid volume (μL)

Internal standard

Number of cycles

Minimum detection limit (copies/mL)

Target genes

Test result positive

Maccura kit

20

Yes

40

450

ORF1ab / N / E

ORF1ab gene

Sigmoidal amplification curve and Ct ≤ 38

N gene

Sigmoidal amplification curve and Ct ≤ 38

E gene

Sigmoidal amplification curve and Ct ≤ 37

 

 + 

 + 

 + 

positive

 + 

 + 

−

positive

 + 

−

 + 

positive

Sansure kit

20

Yes

45

200

ORF1ab / N

ORF1ab gene

Sigmoidal amplification curve and Ct ≤ 40

N gene

Sigmoidal amplification curve and Ct ≤ 40

  

 + 

 + 

 

positive

 + 

−

 

positive

−

 + 

 

positive

  1. Ct Cycle threshold