Fig. 2

Infection titers of 10 lots of CNE2-EBV-GFP virus on Akata cells determined by FCM and HCIS. A Infection titers of 10 lots of CNE2-EBV-GFP virus determined by FCM. Data are shown as mean ± SEM (n = 2). Curves were fit using four parameters nonlinear regression. B Infection titers of 10 lots of CNE2-EBV-GFP virus determined by HCIS. Data are shown as mean ± SEM (n = 2). Curves were fit using four parameters nonlinear regression. One spot represents one cell and GFP positive spots correspond to infected cells. C Half maximal infection dilution fold (ID₅₀) of 10 lots of virus defined by FCM and HCIS, respectively. Horizontal bars indicate mean ± SEM. D Correlation of ID₅₀ values defined between FCM and HCIS for each of the 10 lots of CNE2-EBV-GFP