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Fig. 1 | Virology Journal

Fig. 1

From: A high-throughput neutralizing assay for antibodies and sera evaluation against Epstein-Barr virus

Fig. 1

CNE2-EBV-GFP virus production and TEM detection. A Images of CNE2-EBV cells carrying Akata-EBV-GFP before induction. Few GFP spots were observed indicating that most viruses remained in a latent state. B Images of CNE2-EBV cells carrying Akata-EBV-GFP 72 h after induction. The majority of cells displayed GFP expression, indicating that EBV was induced to switch to a lytic phase of replication. For HCIS quantification, each GFP expressing cell is counted as a positive spot. (A-B). Images of cells with the same field of view captured at FITC channel (left panel) and bright-field channel (right panel). C TEM images of CNE2-EBV-GFP virus. Capsids are visible, but the viral envelope is not observed because sample processing for TEM observation disrupted this membrane structure. The magnification of the left channel is 150,000 x (scale bar: 100 nm) and that of the right channel is 250,000 × (scale bar: 50 nm)

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