Fig. 2

Knockdown of TRIM46 inhibits H7N9/ZJU-1 replication. A549 cells were transfected with negative control sequences and TRIM46 knockdown sequences, TRIM46#1 and TRIM46#2, for 72 h, cells were harvested, the protein levels of TRIM46 were measured by western blotting (A) and the mRNA levels were measured by RT-qPCR (B). C. A549 cells were transfected with TRIM46 shRNA sequence or negative control sequences for 72 h, infected with H7N9/ZJU-1 for 12 h, the cells were lysed and subjected to western blotting with indicated antibodies. TRIM46 Knockdown cells and negative control cells were infected with H7N9/ZJU-1 for 12 h, the relative levels of NP mRNA, cRNA and vRNA were measured by RT-qPCR (D). The supernatant of TRIM46 knockdown cells and negative control cells was collected and viral titers were determined by TCID₅₀ method (E)