Fig. 2
From: A high-throughput screening assay to identify inhibitory antibodies targeting alphavirus release
Generation of CHIKV 181/25 E2nLuc and MAYV E2nLuc reporter viruses. BHK-21 cells were infected with CHIKV/MAYV wild-type (WT) or nLuc reporter virus. Supernatant was harvested 6, 12, 18, and 24 hpi (hours post infection) and used to determine A infectious titres via plaque forming unit assay in U2OS cells, B relative viral RNA levels via qRT-PCR and C nLuc activity (represented by RLU) associated with released viral particles. Graphs represent the arithmetic mean ± SD of three independent experiments. Plaque size was compared for WT and nLuc CHIKV/MAYV with examples of plaques (D) and by quantitating 53 plaques from each virus as described in the methods (E)