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Fig. 1 | Virology Journal

Fig. 1

From: A high-throughput screening assay to identify inhibitory antibodies targeting alphavirus release

Fig. 1

181/25 E2nLuc virus particles carry nano-luciferase activity. A Illustration of CHIKV wildtype and nLuc reporter virus genomes. DNA encoding the nLuc reporter (highlighted) was inserted into viral cDNA clones immediately downstream of the furin cleavage site between E3 and E2. B Gradient-purified 181/25 and 181/25 E2nLuc viral particles underwent three-fold serial dilutions and nLuc activity was measured. Gradient-purified particles were also separated on a SDS-PAGE followed by C Coomassie staining and immuno-blot with D anti-CHIKV and E anti-nLuc

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Virology Journal

ISSN: 1743-422X