Fig. 4

HPV16 E7 increases TLS pathway activation by binding RB. Representative immunoblot of vector control (LXSN) and HPV16 E7 wild type and mutant E7 expressing HFKs probed for A replication stress-responsive proteins and TLS proteins and B densitometry of these proteins calculated from three individual immunoblots. The bars represent the mean of densitometry from at least three independent repeats. As a result, they may not perfectly match the representative blot shown in A. Error bars denote standard errors of the mean. # denote a statistical difference between HPV16 E7 wild type and HPV16 E7 mutant (#− p < 0.05, ##− 0.01). *denotes that there is a statistical difference between LXSN HFKs and the indicated cell line (*− p < 0.05, **− 0.01, ***− 0.001). GAPDH was used as a loading control