Molecular characteristics of a coxsackievirus A12 strain in Zhejiang of China, 2019

Table 3 Amino acid variation sites in VP1 and VP4 of full-length coxsackievirus A12 (CV-A12) PJ201984, CV-A12 prototype strain Texas-12, and consensus CV-A12 sequence

Protein	Variation sites	PJ201984	Texas-12	Consensus CV-A12*
VP1	3	A	T	T
	17	N	S	N
	27	S	T	T
	35	N	T	N
	61	S	N	S
	71	K	R	K
	91	V	I	V
	93	V	I	V
	143	N	S	N
	149	L	M	L
	177	I	V	I
	233	M	I	M
	247	V	I	V
	290	T	A	T
	294	A	S	A
VP4	9	K	R	K
	10	S	T	S
	17	T	N	T
	18	F	I	F
	20	S	T	S
	51	S	N	S
	57	M	I	I
	64	T	V	T

*The consensus CV-A12 sequence was defined as a combination of the majority amino acid in each site, generated by MegAlign
The bolded rows indicated the differences between the PJ201984 and the consensus CV-A12 sequence

Protein	Variation sites	PJ201984	Texas-12	Consensus CV-A12*
VP1	3	A	T	T
	17	N	S	N
	27	S	T	T
	35	N	T	N
	61	S	N	S
	71	K	R	K
	91	V	I	V
	93	V	I	V
	143	N	S	N
	149	L	M	L
	177	I	V	I
	233	M	I	M
	247	V	I	V
	290	T	A	T
	294	A	S	A
VP4	9	K	R	K
	10	S	T	S
	17	T	N	T
	18	F	I	F
	20	S	T	S
	51	S	N	S
	57	M	I	I
	64	T	V	T

Protein	Variation sites	PJ201984	Texas-12	Consensus CV-A12*
VP1	3	A	T	T
	17	N	S	N
	27	S	T	T
	35	N	T	N
	61	S	N	S
	71	K	R	K
	91	V	I	V
	93	V	I	V
	143	N	S	N
	149	L	M	L
	177	I	V	I
	233	M	I	M
	247	V	I	V
	290	T	A	T
	294	A	S	A
VP4	9	K	R	K
	10	S	T	S
	17	T	N	T
	18	F	I	F
	20	S	T	S
	51	S	N	S
	57	M	I	I
	64	T	V	T

Protein	Variation sites	PJ201984	Texas-12	Consensus CV-A12*
VP1	3	A	T	T
	17	N	S	N
	27	S	T	T
	35	N	T	N
	61	S	N	S
	71	K	R	K
	91	V	I	V
	93	V	I	V
	143	N	S	N
	149	L	M	L
	177	I	V	I
	233	M	I	M
	247	V	I	V
	290	T	A	T
	294	A	S	A
VP4	9	K	R	K
	10	S	T	S
	17	T	N	T
	18	F	I	F
	20	S	T	S
	51	S	N	S
	57	M	I	I
	64	T	V	T