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Fig. 3 | Virology Journal

Fig. 3

From: Effects and mechanism of Aβ1−42 on EV-A71 replication

Fig. 3

1–42 targeted the attachment and post-attachment phases of EV-A71 infection. a Time-of-addition experiment. SH-SY5Y cells were subjected to EV-A71 infection (MOI = 10) at 0 h time point. At 1 h point, cells were cleaned in PBS buffer and collected at 8 hpi. EV-A71 VP1 was determined by WB assay. The grey column indicates the period in which 30 µg/mL Aβ1–42 peptides were present. b EV-A71 virus (MOI = 2.5) was added to precooled cell plates simultaneously with Aβ1–42 (30 µg/mL) or pirodavir (40 µM) at 4 °C for 1 h. The amounts of cell-bound EV-A71 particles were measured by qRT-PCR. c Precooled cells that adhered to EV-A71 were incubated with Aβ1–42 (30 µg/mL) or NH4Cl (40 µM) at 37 °C for 60 min. The content of vp1 RNA was identified by qRT-PCR

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