Fig. 6

Specificity and sensitivity of the RT-RAA-Cas12a-based fluorescence and lateral flow strip (LFS) assay. a–d Specificity of RT-RAA-Cas12a-mediated real-time and end-point fluorescence and LFS assay was conducted with human rotavirus (HRV), astrovirus (HAtV), and enterovirus 71 (HEV71) at concentrations of 200 copies/μL. Visualization of Cas12a assay on NOV VP1 in real-time (a), end-point (b), under ultraviolet (UV) light (c) and by LFS (d). (e–h) Sensitivity was conducted with a gradient concentration of NOV standard RNA ranging from 200 to 0.05 copies/μL. Visualization of Cas12a assay on NOV VP1 in real-time (e), end-point (f), under ultraviolet (UV) light (g) and by LFS (h), respectively. Three replicates were conducted for each sample. Error bars indicate the standard deviations of three replicates (n = 3). Statistical analysis was employed to evaluate the difference between detection groups to NC. *p < 0.05; **p < 0.01; ***p < 0.001. NC, the negative control. Ctrl is the control line. Test refers to the test line