Fig. 4

Screening of optimal crRNA for RT-RAA-Cas12a-based assay. The RT-RAA-Cas12a-based real-time (a) and end-point (b) fluorescence readouts were measured after incubation with 30 nM of different crRNA for 15 min and 14 min at 39 °C, respectively. Three replicates were performed for each sample. Error bars refer to the standard deviations at three replicates (n = 3). Statistical analysis was applied to evaluate the difference between test groups to NC. *p < 0.05; **p < 0.01; ***p < 0.001. NC, negative control. c Visualization of RT-RAA-Cas12a-based detection under ultraviolet (UV) light