Fig. 2
Assessment of primer pairs for reverse transcription recombinase-aided amplification (RT-RAA). a The optimal primer pairs for RT-RAA were evaluated with RNA standard of NOV GII.4 or GII.17 at 1 × 105 copies/μL, respectively. Lane 1: NOV-F1/NOV-R1; Lane 2: NOV-F2/NOV-R1; Lane 3: NOV-F1/NOV-R2; Lane 4: NOV-F2/NOV-R2. b Specificity of RT-RAA assay was conducted with three gastrointestinal viruses, including human rotavirus (HRV), astrovirus (HAtV), and enterovirus 71 (HEV71), and the results were demonstrated by agarose electrophoresis. NC, negative control. c Sensitivity of RT-RAA assay was performed using the concentration of RNA standard of NOV GII.4 and GII.17 ranging from 1 × 105 to 1 × 102 copies/μL