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Fig. 6 | Virology Journal

Fig. 6

From: Detection and discovery of plant viruses in soybean by metagenomic sequencing

Fig. 6

Transmission electron micrographs showing the ultrastructure of N. benthamiana mock-inoculated and ClYVV (IA-2017 isolate) infected leaf tissues A Mock inoculated leaf tissue at 10 dpi. Uninfected phloem parenchymal cell (PPC) surrounded by mesophyll cells (MC). Bar = 2 μm. (B-F) ClYVV infected leaf tissue at 10 dpi. B Infected vascular bundle surrounded by mesophyll cells (MC). Crystalline nuclear inclusions (NI) and cylindrical cytoplasmic inclusions (CI) distribution in the phloem cell parenchyma (PCP). Bar = 5 μm. C Infected mesophyll cells (MC). Crystalline nuclear inclusions (NI) and cylindrical cytoplasmic inclusions (CI) distribution in adjacent mesophyll cells. Bar = 2 μm. D Infected vascular parenchymal cell (VPC) above a xylem element (XE) and mesophyll cell (MC). Crystalline nuclear inclusions (NI) distribution in the nucleolus (Nu) and cytoplasm (Cy). Cylindrical cytoplasmic inclusions (CI) distribution in the cytoplasm. Bar = 2 μm. E Magnified virus particles from section of panel D. Virions (Vi), crystalline nuclear inclusions (NI) and cylindrical cytoplasmic inclusions (CI) distribution in the cytoplasm (Cy). Bar = 500 nm. F Magnified virus particles from section of panel D. Crystalline nuclear inclusions (NI) and cylindrical cytoplasmic inclusions (CI) distribution in the cytoplasm (Cy). Bar = 500 nm. C, chloroplast; St, starch; M, mitochondria; RER, rough endoplasmic reticulum; N, nucleus; NE, nuclear envelope; V, vacuole; CW, cell wall; PM, plasma membrane, PD, plasmodesmata; ICS, intracellular space; PC, phloem cell; Asterisk (*), phloem secondary cell wall thickening

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