Fig. 2

Binding of HDV-I, HDV-II, and HDV-III HDAg-L to CHC. A Coimmunoprecipitation of CHC with Flag-tagged HDAg-L. Lysates of Huh7 cells cotransfected with plasmids encoding HBsAg and HDV-I, HDV-II, or HDV-III HDAg-L were incubated with the anti-CHC antibody for precipitation. Levels of CHC, Flag-tagged HDAg-L, and small HBsAg were determined by western blot analysis by using anti-CHC, anti-Flag and anti-HBsAg antibodies. B Pull-down of endogenous CHC with GST-tagged C-terminal HDAg-L. GST-tagged proteins were incubated with the Huh7 cell lysates. Levels of CHC were determined by western blot analysis by using anti-CHC antibody. Coomassie blue staining of GST fusion proteins is shown in the bottom panel. C Pull-down of His-CHC(1–107) with GST-tagged C-terminal HDAg-L. GST-tagged proteins were incubated with the purified recombinant His-CHC(1–107). Levels of His-CHC(1–107) were determined by western blot analysis using anti-His antibody. Coomassie blue staining of GST fusion proteins is shown in the bottom panel. Experiments were performed in triplicate. Results of western blots were quantified by densitometry analysis