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Fig. 1 | Virology Journal

Fig. 1

From: Identification of a novel interaction site between the large hepatitis delta antigen and clathrin that regulates the assembly of genotype III hepatitis delta virus

Fig. 1

Production of HDV-I, HDV-II and HDV-III VLPs. Secretion of HDV-I, HDV-II, and HDV-III VLPs from Huh7 cells in the presence of small HBsAg. HDV VLPs were harvested from the medium of Huh7 cells cotransfected with pECE-C-ES and pCMV-Tag2C-HDAgL, pCMV-Tag2C-HDAgL-II, or pCMV-Tag2C-HDAgL-III at day 4 posttransfection. Levels of Flag-tagged HDAg-L and small HBsAg were detected by western blot analysis using anti-Flag and anti-HBsAg antibodies. Experiments were performed in triplicate. Results of western blots were quantified by densitometry analysis. Protein quantification is shown in the bottom panel

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