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Fig. 3 | Virology Journal

Fig. 3

From: The envelope protein of Zika virus interacts with apolipoprotein E early in the infectious cycle and this interaction is conserved on the secreted viral particles

Fig. 3

ApoE and Zika E protein interact in ZIKV-induced ER rearrangements in infected HMC3 cells. a Western-blot analysis of the co-immunoprecipitation of ApoE and Zika E protein after 48 h post-infection. NI: naive cells; Inf: infected cells; IP: immunoprecipitation; anti-apoE: goat anti-ApoE Abs; anti-gp120: anti-HIV-1 gp120 Abs used as an isotype control for immunoprecipitation assays. b Assay of the colocalization of the ApoE-Zika E proteins with calnexin after 24 h of infection; Pearson coefficient for colocalization within the ROI in HMC3 cells performed on 30 z-stacks. Scale bars = 8 µm. c Characteristic membrane rearrangements were observed in infected HMC3 cells by TEM. White triangles: convoluted membranes; bold black arrow: vesicle packets. Scale bars = 500 nm. d Immunogold labeling of ApoE and Zika E protein with 10 nm and 6 nm gold-conjugated Abs, respectively, in infected HMC3 cells analyzed on cryo-TEM sections. Scale bar = 200 nm

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Virology Journal

ISSN: 1743-422X