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Fig. 5 | Virology Journal

Fig. 5

From: Preparation of the luciferase-labeled antibody for improving the detection sensitivity of viral antigen

Fig. 5

LODs of Nluc-ch2C5 and HRP-ch2C5. A: LODs of Nluc-ch2C5 and HRP-ch2C5 for S-RBD in the indirect ELISA. The S/C values (left); LOD of Nluc-ch2C5 (middle); LOD of HRP-ch2C5(right). B: LODs of Nluc-ch2C5 and HRP-ch2C5 for S-RBD in the double-antibody sandwich assay. S/C values (left); LOD of Nluc-ch2C5(middle); LOD of HRP-ch2C5(right). C: LODs of Nluc-ch2C5 and HRP-ch2C5 for SARS-CoV-2 in the double-antibody sandwich assay. S/C values (left); LOD of Nluc-ch2C5 (middle); LOD of HRP-ch2C5 (right). S/C is the value of the average luminance intensity for the test group divided by the cutoff value (average luminance intensity plus three standard deviations). The dotted line denotes S/C = 1 (in left figures). The inner line is a Probit curve (does-response rule) and the outer dotted/dashed lines are the 95% confidence intervals(in middle and right figures)

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Virology Journal

ISSN: 1743-422X