Fig. 2

A Schematic diagram of the cartridge, which comprises three areas: a virus incubation area, a washing area, and a detection area. B Schematic illustration of the AMCA. (a) 200 μl of sample, 5 μl of magnetic beads (10 mg/ml), and 150 μl of Nluc-ch2C5 (10 ng/ml) were mixed in virus incubation area and incubated for 45 min at 37℃. The mixture is agitated every 15 min. (b) The magnetic bar pulls the magnetic beads into the washing area and the beads are washed four rounds. (c) The magnetic beads are released into the detection area (Nano-Glo substrate). (d) Luciferase intensity is measured