Fig. 1

Flow chart diagram. RNA expression levels in peripheral blood mononuclear cell (PBMC) of dengue fever patients will change during Dengue virus (DENV) infection. We downloaded these RNA-sequence data and clinical information from National Center of Biotechnology Information (NCBI) dataset (https://www.ncbi.nlm.nih.gov/geo/). Combining R package “Limma” with “WGCNA”, stable differentially expressed genes (DEGs) among three phases and between DHF and DF for Dengue patients were screened and we also explored potential functions for these DEGs using Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Set Enrichment Analysis (GSEA) analysis. Based on gene expression data, fractions of immune cells in whole blood samples were estimated by the “CIBERSORT” website and correlations between immune cells and huh genes were also qualified. Finally, we used Area Under the Curve (AUC) to evaluate diagnosis value of huh genes and important immune cells in distinguishing clinical stages and severity