Fig. 7

The phosphatase activity of PP2A was not affected by HCV NS5B. A Huh7.5 cells were transfected with Flag-WT-NS5B, Flag-D559G-NS5B, or control EGFP for 36 h, and then the cells were lysed and subjected for phosphatase activity assay. Y axis shows the absolute value of DiFMU present in the solution determined by fluorescence reads. B The core enzyme catalytic subunit C (PP2Ac, alpha and beta isoforms) was not changed by HCV NS5B. Huh7.5 cells were transfected with Flag-WT-NS5B, Flag-D559G-NS5B, or control EGFP for 36 h, and then the cells were lysed and analyzed by western blot; the levels of PP2Ac (α and β isoforms) were normalized to GAPDH