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Fig. 2 | Virology Journal

Fig. 2

From: PPP2R5D promotes hepatitis C virus infection by binding to viral NS5B and enhancing viral RNA replication

Fig. 2

Co-IP of PPP2R5D with HCV WT-NS5B and D559G-NS5B. 293T cells were co-transfected with HA-tagged PPP2R5D and Flag-tagged WT-NS5B or D559G-NS5B, and the cells were lysed 48 h post-transfection and subjected to immunoprecipitation experiment. (A) Co-IP was performed using anti-HA antibody and immunoblotted (IB) with anti-HA or anti-Flag antibodies. The amount of D559G-NS5B pulled down by PPP2R5D was slightly more than that of WT-NS5B. (B) Co-IP was performed using anti-Flag antibody and immunoblotted with anti-HA or anti-Flag antibodies

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Virology Journal

ISSN: 1743-422X