Fig. 1

Ectopic expression and pull-down assay of D559G-NS5B and wild-type (WT)-NS5B. A Plasmids expressing Flag-tagged WT-NS5B and D559G-NS5B were transfected into 293T cells for 24 h, and NS5B expression was detected by western blot using anti-Flag antibody. Plasmid expressing EGFP was used as transfection control, and β-actin was detected as an internal control. B Silver staining of PAGE after separation of Flag-tagged NS5B-pull down mixtures. A band was visualized with more intensity in D559G-NS5B pellet (boxed), which was sliced and sent for mass spectrometry analysis, together with the gel slice from same position of WT-NS5B lane