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Fig. 5 | Virology Journal

Fig. 5

From: Enhanced synergistic antitumor effect of a DNA vaccine with anticancer cytokine, MDA-7/IL-24, and immune checkpoint blockade

Fig. 5

Characterization of depletion of CD4+ CD25+ Foxp3+ T cells in immunized mice. A–E Representative dot plots data demonstrating the frequency of CD4+ CD25+ Foxp3+  lymphocytes in the splenocytes of various immunized mice groups. Splenocytes were collected one week after last intratumoral injection of DNA vaccine and adjuvant, then subjected to flow cytometry analysis using the anti-CD4, anti-CD25, and anti-Foxp + antibodies. F Bar figures represent the percentages of CD4+ CD25+ Foxp3+ T cells (Treg) among CD4+ T cells from splenocytes. Mice treated with DNA E7 plus IL-24 had the lowest numbers of Treg cells from splenocytes among the other groups (*** p < 0.001). Data demonstrate mean ± one standard error of two independent experiments

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Virology Journal

ISSN: 1743-422X