Fig. 4

Levels of inflammatory mediators secreted by splenocytes in immunized mice. The supernatants of splenocytes were re-stimulated with E7 specific epitope and collected one week after the last injection. ELISA method was performed to determine the level of IFN-γ (A), IL-4 (B), TNFα (C) and IL-10 (D) in splenocyte cultures. Results are representative of three independent experiments and are expressed as the mean ± SD. The DNA E7 adjuvanted with IL-24 induced robust levels of IFN-γ, IL-4 and TNFα when compared to the other groups (A–C). Also, level of IL-10 (D) in DNA E7 adjuvanted with the IL-24 group was significantly reduced compared with other groups (IL-24, pcDNA, PBS). *P < 0.05; **P < 0.01; ***P < 0.001