Fig. 5

Effects of MHBSt¹⁶⁷-induced NF-κB activation on cell proliferation and the cell cycle in L02 cells. A Western blotting analysis of NF-κB activation and statistical analysis. Before transfection with MHBS and MHBSt¹⁶⁷ expression plasmids, cells were treated with DMSO and 0.3 µM BAY-11-7082 for 2 h. The individual gray value in Western blots was measured and normalized against β-actin, then the relative intensity of target protein to β-actin was calculated by setting the control vector transfection as 1.00, *P < 0.05. B Cell viability was measured by CCK-8 assay in L02 cells. Before transfection with MHBS and MHBSt¹⁶⁷ expression plasmids, cells were treated with DMSO and 0.3 µM BAY-11-7082 for 2 h. The results are expressed as the OD values. *P < 0.05. C–E Cell cycle analysis was performed by flow cytometry. Cells were harvested after being transfected with plasmids for 48 h and stained with PI. Representative images from three independent experiments are shown