Fig. 4

Effects of MHBSt¹⁶⁷ expression on NF-κB activation in L02 cells. A Western blotting analysis of NF-κB activation and statistical analysis. The individual gray value in Western blots was measured and normalized against β-actin, then the relative intensity of target protein to β-actin was calculated by setting the control vector transfection as 1.00, *P < 0.05. B Confocal microscopic analysis of NF-κB activation. The intracellular localization of p-NF-κB/p65 was determined by immunofluorescence staining using an anti-p-NF-κB/p65 antibody. Nuclei were stained with DAPI. The red fluorescence intensity in unclear was analyzed by Image J. *P < 0.05. Scale bar: 50 μm. C RT-PCR analysis of IFN-α, IFN-β and IL-1α in cells. **P < 0.01. Representative images from three independent experiments are shown