Skip to main content
Fig. 2 | Virology Journal

Fig. 2

From: MHBSt167 induced autophagy promote cell proliferation and EMT by activating the immune response in L02 cells

Fig. 2

Effects of MHBSt167 expression on autophagy in L02 cells. A Western blotting analysis of autophagy-related proteins (LC3B, Beclin1, and p62) and statistical analysis. Cells were treated with DMSO, 100 nM rapamycin (Rapa), or 4 mM chloroquine (CQ) for 2 h and then transfected with MHBS and MHBSt167 expression plasmids for 48 h. The individual gray value in Western blots was measured and normalized against β-actin, then the relative intensity of target protein to β-actin was calculated by setting the control vector transfection as 1.00, *P < 0.05. B Confocal microscopic analysis of LC3B puncta induced by MHBSt167 via immunofluorescence staining. An exogenous GFP-LC3 expression plasmid was transfected into L02 cells. Nuclei were stained with DAPI. The LC3-puncta per cell was analyzed. C Confocal microscopic analysis of autophagic lysosome formation induced by MHBSt167. An exogenous GFP-LC3 expression plasmid was transfected into L02 cells. Before immunofluorescence staining, the cells were incubated with 50 nM LysoTracker Red for 2 h. The LC3/LysoTracker-positive puncta per cell was analyzed. D Confocal microscopic analysis of autophagy flux induced by MHBSt167. An exogenous RFP-GFP-LC3 expression plasmid was transfected into L02 cells. The ratio of GFP/RFP–positive puncta number per cell was analyzed. The ratio in cells transfected with Vector was defined as 1. Scale bar: 50 μm. Representative images from three independent experiments are shown

Back to article page