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Fig. 3 | Virology Journal

Fig. 3

From: Identification and application of a pair of noncompeting monoclonal antibodies broadly binding to the nucleocapsid proteins of SARS-CoV-2 variants including Omicron

Fig. 3

The abilities of P301-F7 and P301-H5 in recognizing the mutated NPs and live viruses of SARS-CoV-2 variants. ELISA analysis of the binding activities of P301-F7 and P301-H5 to the SARS-CoV-2 Omicron NP (a) and the mutated NPs (b) carrying D3L/S235F, P13L, S194L, R203K/G204R, and I292T substitutions, respectively. The pAb is used here as a positive control. c Key mutations appear in the NP of isolated SARS-CoV-2 live viruses used in this study. d The immune spot assay analysis of the recognition abilities of P301-F7 to the WT SARS-CoV-2 and variants (Alpha, Beta, Delta, and Omicron). The Vero E6 cells were infected with SARS-CoV-2 live virus, respectively, and then stained by the HRP-labeled P301-F7. The experiment in a, b, d was performed twice and one representative result was shown

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Virology Journal

ISSN: 1743-422X