Table 1 Primer sequences for qPCR assays
From: Anti-L1 antibody-bound HPV16 pseudovirus is degraded intracellularly via TRIM21/proteasomal pathway
Primers | Sequence | Used for |
|---|---|---|
P1 | F: 5′-CGAGTGACAAGCCTGTAGCCC-3′ | TNF-α |
P2 | R: 5′-CCTTGAAGAGGACCTGGGAGT-3′ | |
P3 | F: 5′-GAGCAAGGACCCTCACGACC-3′ | IRF-3 |
P4 | R: 5′-GGCCAACACCATGTTACCCAGT-3′ | |
P5 | F: 5′-ACCAGTCCATCCCAGTGGCT-3′ | IRF5 |
P6 | R: 5′-TTCGGTGTATTTCCCTGTCTCC-3′ | |
P7 | F: 5′-TACCTAGAGTACCTCCAGAACAG-3′ | IL-6 |
P8 | R: 5′-CATTTGCCGAAGAGCCCTCA-3′ | |
P9 | F: 5′-GCAGCTGAGAATCCTGGG-3′ | TRIM21 |
P10 | R: 5′-CAGCCCTGGCACATGGC-3′ | |
P11 | F: 5′-TCGTGACCACCCTGACCTACGGCGTG-3′ | eGFP |
P12 | R: 5′-CACCTTGATGCCGTTCTTCTGCTTGTCG-3′ |