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Table 1 The sequences of primers used for construction of porcine HnRNP F (GenBank: XM_005671001.3) mutants plasmids

From: Interaction of HnRNP F with the guanine-rich segments in viral antigenomic RNA enhances porcine reproductive and respiratory syndrome virus-2 replication

Name

Primer Sequence (5'-3')

HnRNP F-F

TTTGGATCCATGATGTTGGGCCCTGAGGGAGGTGAAG

HnRNP F-R

TTTCTCGAGCTAGTCATATCCACCCATGCTGTTCTGG

ΔqRRM1-F

TTTGGATCCTTCAAGTCCCACAGAACCGAGATGGAT

ΔqRRM2-F

GACACTGCCAATGATGAAGAGGTTAGGTCATACTC

ΔqRRM2-R

TGACCTAACCTCTTCATCATTGGCAGTGTCTGCAC

ΔqRRM3-R

TTT CTCGAGTTATCCAGTGGTGCTCTGGACAGTAAACTCG

qRRM3-F

TTTGGATCCGAAGAGGTTAGGTCATACTCAGATCC

  1. The restriction enzymes BamH I and Xho I cutting sites used for cloning are underlined in italics
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Virology Journal

ISSN: 1743-422X