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Fig. 6 | Virology Journal

Fig. 6

From: Interaction of HnRNP F with the guanine-rich segments in viral antigenomic RNA enhances porcine reproductive and respiratory syndrome virus-2 replication

Fig. 6

The formation of N–HnRNP F–DHX36 complexes following PRRSV infection. a Formation of a putative trimeric complex between PRRSV N protein, HnRNP F, and DHX36 in HEK293T cells transfected with HA-N protein, and Flag-HnRNP F. Lysates were immunoprecipitated and analyzed by western blotting with indicated antibodies. b Assessment of the binding of endogenous HnRNP F and DHX36 to PRRSV N protein in PRRSV infected cells using Co-IP. c Wild-type HA-N protein was transiently co-expressed with the indicated full-length porcine Flag-HnRNP F or variants in HEK293T cells, precipitated using anti-Flag antibody and co-precipitation of N protein and DHX36 were detected by western blotting. d HEK293T cells were transfected with Flag-HnRNP F and its truncation mutants. 36 h later, Co-IP was performed to analyze the interaction between DHX36 and HnRNP F mutants

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Virology Journal

ISSN: 1743-422X