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Fig. 1 | Virology Journal

Fig. 1

From: Interaction of HnRNP F with the guanine-rich segments in viral antigenomic RNA enhances porcine reproductive and respiratory syndrome virus-2 replication

Fig. 1

HnRNP F knockdown impairs PRRSV replication. a Endogenous HnRNP F mRNA levels were examined by real-time PCR in negative control-siRNA (NC) or si-HnRNP F treated PAMs to confirm the knockdown efficiency. b The toxicity of HnRNP F depletion toward PAMs after transfection was tested using an Enhanced Cell Counting Kit-8. c–e PAMs were transfected with the indicated siRNAs, and 24 h later were infected with PRRSV (MOI = 0.1). The cells were collected at 24–36 hpi to determine total viral RNA levels by real-time PCR (c), N protein expression levels by western blotting assays (d), and viral titers in supernatants by plaque assay (e), respectively

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Virology Journal

ISSN: 1743-422X