Development of a multiplex droplet digital PCR assay for detection of enterovirus, parechovirus, herpes simplex virus 1 and 2 simultaneously for diagnosis of viral CNS infections

Table 1 Sequences of primers and probes

Primer/probe	Sequence (5′–3′)	Concentration, nmol/L		Refs.
Primer/probe	Sequence (5′–3′)	Simplex ddPCR	Quadruplex ddPCR	Refs.
EV-for^a	GGTGTGAAGAGCCTATTGAGCT	400	400	In house
EV-rev^b	GAAACACGGACACCCAAAGTAGT	400	400
EV-probe	FAM-TCCTCCGGCCCCTGAATGCGGCTAATCC-BHQ1	200	200
HSV2-for	CGCTCTCGTAAATGCTTCCCT	400	400	[23]
HSV2-rev	TCTACCCACAACAGACCCACG	400	400
HSV2-probe	FAM-CGCGGAGACATTCGAGTACCAGATCG-BHQ1	–	100
	VIC-CGCGGAGACATTCGAGTACCAGATCG-BHQ1	200	100
HPeV-for	GTGCCTCTGGGGCCAAAAG	400	400	[24]
HPeV-rev	TCAGATCCATAGTGTCGCTTGTTAC	400	400
HPeV-probe	FAM-ACGGGTACCTTCTGGGCATCCTTCG-BHQ1	200	50
	VIC-ACGGGTACCTTCTGGGCATCCTTCG-BHQ1	–	150
HSV1-for	CGGCCGTGTGACACTATCG	400	400	[23]
HSV1-rev	CTCGTAAAATGGCCCCTCC	400	400
HSV1-probe	VIC-CCATACCGACCACACCGACGAAC-BHQ1	200	400

ISSN: 1743-422X