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Fig. 2 | Virology Journal

Fig. 2

From: Development of a multiplex droplet digital PCR assay for detection of enterovirus, parechovirus, herpes simplex virus 1 and 2 simultaneously for diagnosis of viral CNS infections

Fig. 2

Quadruplex ddPCR assay droplet separation results. A is a two-dimensional (2D) amplitude result of quadruplex ddPCR assay. The x-axis is the fluorescence amplitude at Channel VIC (Channel 2, Ch2) and the y-axis stands for channel FAM (Channel 1, Ch1). A, B, and C are 2D plots using different concentrations of nucleic acid mixtures. D and E are two typical 1D plots of quadruplex ddPCR assay and generate from the same reaction of C. The positive droplets located at specific areas represent different positive targets as indicated by the red arrow. F is a representative result of quadruplex ddPCR assay performed on a 105 dilution (high concentration) of the nucleic acid mixture. Blue droplets: positive for EV; red droplets: positive for HSV2; purple droplets: positive for HPeV; green droplets: positive for HSV1; grey droplets: negative; the others (light blue droplets) are positive for one FAM- and one VIC-labeled target at least

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