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Table 5 The clinical performance of the qq-PCR assay for seasonal HCoVs

From: Establishment and evaluation of a quadruple quantitative real-time PCR assay for simultaneous detection of human coronavirus subtypes

  Respiratory pathogen 13 detection Kit Performance characteristics
Positive Negative Sensitivity (%) Specificity (%) Youden's Index PPV (%) NPV (%) Agreement Kappa
qq-PCR assay
 Positive 45 0 95.7 100.0 0.957 100.0 98.0 98.6 0.968 (P < 0.01)
 Negative 2 97        
 Total 47 97        
  1. HCoVs, human coronavirus; PPV, positive predictive value; NPV, negative predictive value