Establishment and evaluation of a quadruple quantitative real-time PCR assay for simultaneous detection of human coronavirus subtypes

Table 3 The correlation coefficients and limit of detection for each gene

Targets	Gene	Correlation coefficients	Limit of detection (copies/μl)
SARS-CoV-2	S	0.998	3.2 × 10¹
SARS-CoV-2	N	0.999	4.8 × 10¹
SARS-CoV	N	0.998	4.8 × 10¹
HCoV-229E	ORF1ab	0.999	3.8 × 10¹
HCoV-NL63	ORF1ab	0.997	6.5 × 10¹
HCoV-HKU1	ORF1ab	0.998	4.2 × 10¹
HCoV-OC43	ORF1ab	0.999	3.7 × 10¹
human RNA	B2M	0.997	2.5 × 10¹

SARS-CoV-2, severe acute respiratory syndrome coronavirus 2; SARS-CoV, severe acute respiratory syndrome coronavirus; HCoV, human coronavirus

ISSN: 1743-422X