Table 1 Sequences of probes and primers
Virus | Primer/probe | Sequence (5′–3′) | Length of amplicons (bp) |
|---|---|---|---|
Rotavirus A | F | ATTGAAGCAGAWTMTGATTCTG | 147 |
R | ACAAATCYTYRATCAATTGCAT | ||
P | VIC-ATTCAGATAGTGATGAYGGWAAATGTA-BHQ1 | ||
Ph | TACATTTCCCTTCATCACTATTTGAAT-Phosphorylation | ||
Rotavirus B | F | CTCAGTTCTTGTAGTATATTAGCTATCG | 133 |
R | CTCCACGAGGCAATTTGATA | ||
P | FAM-AACGCTTCTATGGATTTTAATGTTTTTCTTCAG-BHQ1 | ||
Ph | CTGAATAAAAACTTTAATATCCATATAAGTGTT-Phosphorylation | ||
Rotavirus C | F | CCATTCTCTTCATTCTTTTCATTT | 215 |
R | TGGAAAATAAATACATAAAGATTCAAC | ||
P | FAM-TAATTGTGTAGAGTGGTCACAAGGTCAGATG-BHQ1 | ||
Ph | CATCTGATCTTGTTACCACTTTACATAATTA-Phosphorylation | ||
Norovirus GI | F | TTCCATGATTTGAGTTTGTGG | 100 |
R | GGTGCCATCCATGTTTGTT | ||
P | FAM-CAGGAGACCGCGATCTCCTGC-BHQ1 | ||
Ph | GCATGAGATCTCGTTTTCCTG-Phosphorylation | ||
Norovirus GII | F | AAAGACTGTCTGAAGCTACTCCT | 105 |
R | GACCAGATTTAGTGATAAACACAAT | ||
P | VIC-CTCCCCTGCAAGAGGCCATTT-BHQ1 | ||
Ph | AAATGTCCTTTTGTAGGGTAG-Phosphorylation | ||
Astrovirus | F | AATTGCTCAAAGAGGAAATAGA | 199 |
R | GCAAGTATVCCATTGATTTCAT | ||
P | FAM-AGAACTGCAATGGAACGTGAGATGAAG-BHQ1 | ||
Ph | CTTCTTCTCATGTTCTATTGTAGTTCT-Phosphorylation | ||
Sapovirus | F | CCAATGTCAATTACGACCAG | 115 |
R | TCCATTTCAAACACTAATTTGG | ||
P | VIC-CCACCTACGAATCTTGGTTCATAGGCG-BHQ1 | ||
Ph | CGCTTATGAATCAAGATTCGTATGTGG-Phosphorylation | ||
Adenovirus | F | CTTACAAAGTGCGCTTTACG | 110 |
R | AGGGTTTAAAGCTGGGGC | ||
P | VIC-CATGGCCAGCACCTACTTTGACATC-BHQ1 | ||
Ph | GATTTCAAATTAGTTGCTGTCCTTG-Phosphorylation |