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Fig. 4 | Virology Journal

Fig. 4

From: HBx inhibits DNA sensing signaling pathway via ubiquitination and autophagy of cGAS

Fig. 4

HBx binds to and interacts with cGAS. (a, b) SMMC-7721 cells were transfected with an empty vector or HBx-Flag plasmid. At 24 h posttransfection, cells were harvested, and obtain total protein. We used the IgG and anti-HBx or anti-cGAS antibody to pull the protein, and the interaction was detected by western blot analysis. (c) SMMC-7721 and L02 cells were transfected with an empty vector or HBx-Flag plasmid. At 24 h posttransfection, stain with fluorescent antibody as indicated and observed by fluorescence microscope

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