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Table 4 Evaluation of the agreement between the results by protocol targets using the Cohen’s Kappa agreement coefficient

From: SARS-CoV2 RT-PCR assays: In vitro comparison of 4 WHO approved protocols on clinical specimens and its implications for real laboratory practice through variant emergence

 

Protocol

BERLIN

HKU

DAAN Gene®

IPP

Protocol

Target

BERLIN E

BERLIN RdRp

HKU N

HKU Orf1b

DAAN Gene® N

DAAN Gene® Orf1ab

IPP IP2

IPP IP4

BERLIN

BERLIN E

1

       
 

BERLIN RdRp

0.732

95% CI [0.617–0.848]

1

      

HKU

HKU N

0.713

95% CI [0.591–0.836]

0.668

95% CI [0.539–0.796]

1

     
 

HKU Orf

0.476

95% CI [0.324–0.628]

0.547

95% CI [0.399–0.694]

0.732

95% CI [0.615–0.848]

1

    

DAAN Gene®

DAAN Gene® N

− 0.012

95% CI [− 0.104 to 0.080]

0.022

95% CI [− 0.054 to 0.098]

0.455

95% CI [0.305–0.604]

− 0.041

95% CI [− 0.119 to 0.037]

1

   
 

DAAN Gene® Orf1ab

0.476

95% CI [0.324–0.628]

0.463

95% CI [0.325–0.601]

0.455

95% CI [0.305–0.604]

0.353

95% CI [0.213–0.493]

0.156

95% CI [0.025–0.287]

1

  

IPP

IPP IP2

0.681

95% CI [0.554–0.809]

0.541

95% CI [0.396–0.686]

0.587

95% CI [0.445–0.728]

0.542

95% CI [0.399–0.685]

− 0.036

95% CI [− 0.123–0.051]

0.391

95% CI [0.236–0.545]

1

 
 

IPP IP4

0.240

95% CI [0.102–0.370]

0.011

95% CI [− 0.150 to 0.172]

0.124

95% CI [− 0.025 to 0.274]

− 0.056

95% CI [− 0.219 to 0.106]

− 0.006

95% CI [− 0.060 to 0.048]

0.117

95% CI [− 0.002 to 0.237]

0.312

95% CI [0.169–0.454]

1

  1. Charité, Berlin protocol (BERLIN): Berlin E and Berlin RdRp, University of Hong Kong protocol (HKU): HKU N and HKU Orf 1b, DAAN Gene® protocol (DAAN Gene®): DAAN Gene® N and DAAN Gene® Orf1ab, The Institut Pasteur, Paris (IPP) protocol: IPP IP2 and IPP IP4. 95% CI: Confident interval of 95%, Concordance between two observations increases when Kappa coefficient is closer to 1.bold cases indicate good agreement