Table 1 Description of the obtained RT-PCR results by protocol

“Positive”: target amplification by both targets  per protocol

POSITIVE n(%)

n = 116 (44.6%)

116 (100%)

116 (100%)

n = 112 (43.1%)

112 (100%)

112 (100%)

n = 143

(55%)

143 (100%)

143 (100%)

n = 89

(35%)

89 (100%)

89 (100%)

Ct value

Median [min, max]

29 [16.39]

31 [18.39]

28[12.39]

27.5[16.39]

25[13.39]

28[16.39]

27[15.38]

27[15.39]

Ct value mean +/− SD

28.9 +/− 5.2

30.5 +/− 4.8

27.4 +/− 5.9

27.5 +/− 5.9

26.2 +/− 6.6

28.5 +/− 6.1

27.4 +/− 5.8

27.4 +/− 6.4

MEAN PAIRED DIFFERENCE +/− SD

1.5 +/− 2

0.2 +/− 1.9

2.3 +/− 2

0.04 +/− 1.6

PAIRED SAMPLE t-TEST

p < 0.0001

p = 0.3

p < 0.0001

p = 0.8

« Partial positive»

Target amplification by one target per protocol

POSITIVE n (%)

n = 17 (6.5%)

15 (88.2%)

2 (11.7%)

n = 17 (6.5%)

15 (88.2%)

2 (11.7%)

n = 39

(15%)

37 (94.9%)

2 (5.1%)

n = 44

(16.9%)

38 (86.4%)

6 (13.6%)

Ct value

Median [min, max]

37[24. 39]

33.5[33. 34]

36[31.38]

38[37.39]

38[35.39]

39[39.39]

24.5[15.38]

33.5[32.38]

Ct value mean +/− SD

36 +/− 3.8

33.5 +/− 0.7

36 +/− 1.9

38 +/− 1.4

37.7 +/− 1.2

39 +/− 0

25.2 +/− 6.4

34.3 +/− 2.2

NEGATIVE n (%)

2 (11.7%)

15 (88.2%)

2 (11.7%)

15 (88.2%)

2 (5.1%)

37 (94.9%)

6 (13.6%)

38 (86.4%)

« NEGATIVE»

No target amplification by both targets per protocol, n(%)

n = 127 (48.8%)

127 (100%)

127 (100%)

n = 131. (50.4%)

131 (100%)

131 (100%)

n = 78 (30%)

78 (100%)

78 (100%)

n = 125 (48.10%)

127 (100%)

127 (100%)

Total

260 (100%)

260

(100%)

260

(100%)

260 (100%)

260

(100%)

260 (100%)

260 (100%)

260 (100%)

260 (100%)

260 (100%)

260

(100%)

260

(100%)