Fig. 7

Viral penetration assay. PK-15 confluent cells were pre-cooled and infected with 10 µL of PCV2 suspension (10⁶ TCID₅₀ ml⁻¹, MOI = 5) and incubated at 4 °C for 3 h to allow viral attachment. After this step, the cells were treated at room temperature with 100 µL/well of each polymer using the same four concentrations (0.5,1.0,1.5, and 2.0%), followed by incubation at 37 °C/5% CO₂ for 20, 40, and 60 min (separate plates) to allow the compound to interrupt penetration of virus into cells. Each time point was tested separately and independently. White bar represents virus control. The results are presented as log₁₀ viral DNA copies/mL (panel A) and log₁₀ TCID50/ml (panel B) are expressed as the mean ± SD generated from three replicates and a significant differences set to p ≤ 0.05 and ** p ≤ 0.005 were considered was considered