Fig. 6

Viral attachment assay. Confluent PK15 cell monolayers were pre-cooled at 4 °C for 1 h followed by (1) Cellular infection with 10 µL/well of PCV2 suspension (10⁶ TCID₅₀ ml⁻¹, MOI = 5), supplemented with 100 µl of MEM containing LMW Chi-S at 20 mg/mL (2) infection with 10 µL/well with virus suspension (10⁶ TCID₅₀ ml⁻¹) that had been mixed and pre-incubated with 100 µL/well of the polymer at 4 °C for 30 min. White bar represents virus control. The results are presented as log₁₀ viral DNA copies/mL (panel A) and log₁₀ TCID50/ml (panel B) are expressed as the mean ± SD generated from three replicates and a significant differences set to p ≤ 0.05 and ** p ≤ 0.005 were considered was considered