Fig. 5
From: Oncolytic therapy with vaccinia virus carrying IL-24 for hepatocellular carcinoma
VG9-IL-24 induced apoptosis of hepatocellular carcinoma cells. A SMMC-7721, Hep3B, HepG2 and HL-7702 cells treated with PBS, VG9-IL-24 or VG9-EGFP for 24 h were stained with Hoechst 33258 to visualize morphological changes. Chromatin condensation, nuclear shrinkage, or fragmentation were observed in virus-treated groups but not in normal cells. Bar: 20 μm. B The percentage of apoptotic cells was determined by flow cytometry. SMMC-7721 cells treated with PBS, VG9-IL-24 or VG9-EGFP were harvested after 12 h and stained with FITC-labeled annexin V and PI and immediately analyzed by flow cytometry. Each bar represents the mean ± SD (n = 3). *P < 0.05 versus VG9-EGFP group; **P < 0.01 versus PBS (Control) group