Table 1 Primer pairs used in this study
From: Functional analysis of a viral promoter from a strawberry vein banding virus isolate from China
Name of primers | Sequence of primers | Restriction site |
|---|---|---|
P1/F | 5′-GGATCCGTCATCGCATATGTTCGAGACC-3′ | BamHI |
P1/R | 5′-TCTAGAATGTAAGCAGTTAGGCCCTGTG-3′ | XbaI |
P2/F | 5′-GGATCCCATGGACTCCTTGACTATGTACA-3′ | BamHI |
P2/R | 5′-TCTAGACCGGCAGTTCTTGACTAGGACCT-3′ | XbaI |
P3/F | 5′-GGATCCGTCATCCAAAGAGCACTTAGACC-3′ | BamHI |
P3/R | 5′-TCTAGACGTAGCTACGTACCCCGATGGC-3′ | XbaI |
P4/F | 5′-GGATCC GTCATCGCATATGTTCGAGACC-3′ | EcoRI |
P4/R | 5′-TCTAGAATGTAAGCAGTTAGGCCCTGTG-3′ | BamHI |
USPCH/F-1 | 5′-GGATCCAGAGCACTTCCAAAGA-3′ | BamHI |
USPCH/R-1 | 5′-TCTAGAGTTAGGTAAGCAGCTA-3′ | XbaI |
CHPCH/F1 | 5′-GAATTCGTCATCCAAAGAGCACTTAGACC-3′ | EcoRI |
CHPCH/R1 | 5′-GGATCCCGTAGCTACGTACCCCGATGGC-3′ | BamHI |
CHPCH/F2 | 5′-GAATTCCATGGACTCCTTGACTATGTACA-3′ | EcoRI |
CHPCH/R2 | 5′-GGATCCCCGGCAGTTCTTGACTAGGACCT-3′ | BamHI |
CHPCH/F3 | 5′-GAATTCGTCATCCAAAGAGCACTTAGACC-3′ | EcoRI |
CHPCH/R3 | 5′-GGATCC CGTAGCTACGTACCCCGATGGC-3′ | BamHI |
CHPCH/F4 | 5′-GAATTCGTCATCGCATATGTTCGAGACC-3′ | EcoRI |
CHPCH/R4 | 5′-GGATCCATGTAAGCAGTTAGGCCCTGTG-3′ | BamHI |
USPCH/F-2 | 5′-GAATTCAGAGCACTTCCAAAGA-3′ | EcoRI |
USPCH/R-2 | 5′-GGATCCGTTAGGTAAGCAGCTA-3′ | BamHI |
GUS/F | 5′-CATGGCTGGATATGTATCACCGCGT-3′ | – |
GUS/R | 5′-CGAAGTTCATGCCAGTCCAGCGT-3′ | – |
β-actin/F | 5′-CAATCCAGACACTGTACTTTCTCTC-3′ | – |
β-actin/R | 5′-AAGCTGCAGGTATCCATGAGACTA-3′ | – |