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Fig. 4 | Virology Journal

Fig. 4

From: Functional analysis of a viral promoter from a strawberry vein banding virus isolate from China

Fig. 4

Analysis of GUS activity and accumulation of GUS mRNA in transgenic plants. A GUS activities in the leaves from different transgenic tobacco plants. Leaves were harvested from tobacco plants transformed with pINT-SP1, pINT-FLt-US, pINT121, or pINT-35SΔ (negative control) vector. The harvested leaf tissues were analyzed for GUS activity using fluorometric assays. Five biological replicates were used for each treatment, and the experiment was repeated three times. Standard errors were determined using the LSD method. The biochemical expression assay in leaves was repeated thrice and average readings are represented. Statistical analysis showed a P value of < 0.05, indicating high significance. B Relative expression levels of the GUS gene in different transgenic tobacco plants. Total RNA was extracted from leaves harvested from tobacco plants transformed with the pINT-SP1, pINT-FLt-US, pINT121, or pINT-35SΔ (negative control) vector. Relative GUS mRNA levels were determined by RT-qPCR using GUS gene-specific primers. The relative expression level of the tobacco β-actin gene was used as an internal control. Standard errors were determined using the LSD method. The biochemical expression assay in leaves was repeated thrice and average readings are represented. Statistical analysis showed a P value of < 0.05, indicating high significance

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