Table 1 Primers used for qPCR
Target gene | Direction | Sequence (5′–3′) |
|---|---|---|
β-Actin | Forward | ATCTGGCACCACACCTTCTACAATGAGCTGCG |
β-Actin | Reverse | CGTCATACTCCTGCTTGCTGATCCACATCTG |
RUNX1 | Forward | CTTTCAAGGTGGTGGCCCTA |
RUNX1 | Reverse | CTTGCGGTGGGTTTGTGAAG |
IFNB1 | Forward | TTGTTGAGAACCTCCTGGCT |
IFNB1 | Reverse | TGACTATGGTCCAGGCACAG |
ISG15 | Forward | CGCAGATCACCCAGAAGATCG |
ISG15 | Reverse | TTCGTCGCATTTGTCCACCA |
MxA | Forward | GTTTCCGAAGTGGACATCGCA |
MxA | Reverse | GAAGGGAACTCCTGACAGT |
TRAF3 | Forward | GCGTGTCAAGAGAGCATCGTT |
TRAF3 | Reverse | GCAGATGTCCCAGCATTAACT |
RIG-I | Forward | ACGCAGCCTGCAAGCCTTCC |
RIG-I | Reverse | TGTGGCAGCCTCCATTGGGC |
MAVS | Forward | CAGGCCGAGCCTATCATCTG |
MAVS | Reverse | GGGCTTTGAGCTAGTTGGCA |
TBK1 | Forward | GGCGGCTAGAAGAGGCTTTG |
TBK1 | Reverse | CTCCGTCAGCTCGGTGTAG |
IRF3 | Forward | GCAGGAGGATTTCGGAATCTTC |
IRF3 | Reverse | GGAAATTCCTCTTCCAGGTTGG |
STAT1 | Forward | TGGCCCTAAAGGAACTGGAT |
STAT1 | Reverse | CACTATCCGAGACACCTCGTC |
PR8-NP | Forward | TGCCTGTGTGTATGGACCTG |
PR8-NP | Reverse | TTCAAATGCGGCAGAATGGC |
PR8-M | Forward | CCAGCATCGGTCTCATAGGC |
PR8-M | Reverse | TCGATCCAGCCATTTGCTCC |