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Fig. 4 | Virology Journal

Fig. 4

From: RUNX1 inhibits the antiviral immune response against influenza A virus through attenuating type I interferon signaling

Fig. 4

RUNX1 attenuates PR8-induced IFN-β and ISG expression. a, c, e The shRUNX1 cells and shControl cells were infected with PR8 (MOI = 5) and harvested at 3, 6, and 9 h.p.i. The mRNA level of IFNB1, MxA and ISG15 was assessed by qRT-PCR. b, d, f The A549 cells which were transfected with empty plasmid pCMV-GFP or pCMV-RUNX1. After 24 h transfection, the cells were infected with PR8 (MOI = 5) and harvested at 3, 6, and 9 h.p.i. The mRNA level of IFNB1, MxA, and ISG15 was assessed by qRT-PCR. (g) The shRUNX1 cells and shControl cells were infected with influenza A/Zhejiang/163/2020 (H3N2) (ZJ163), A/swine/Jiangsu/C1/2008 (H9N2) (JSC1) and A/California/04/2009(H1N1) (CA04) (MOI = 1) and harvested at 9 h.p.i. The mRNA level of IFNB1, MxA and ISG15 in the A549 cells was assessed by qRT-PCR. h The mRNA level of IFNB1, MxA, and ISG15 in the shRUNX1 cells and shControl cells without viral infection was assessed by qRT-PCR. i The mRNA level of IFNB1, MxA, and ISG15 in the A549 cells which were transfected with empty plasmid pCMV-GFP or pCMV-RUNX1 was assessed by qRT-PCR. Data are mean ± SD of three independent experiments. Significance is by unpaired T-test; *p < 0.05; **p < 0.01. ***p < 0.001

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